Digitales Projekttreffen 30.9.21 - 01.10.2021


Wir veranstalten unser erstes digitales Projekttreffen vom 30.09.21 bis 01.10.21. Hier werden alle Projektteilnehmer*innen virtuell zusammen kommen und erste Ergebnisse präsentieren. Das Treffen soll vor allem den jungen Wissenschaftler*innen (Doktorand*innen, Postdoktorand*innen), die an den Projekten arbeiten, die Möglichkeit geben, sich und ihr Projekt zu präsentieren.


Thursday, September 30, 2021:

09:00 – 09:10 Gabriele Pradel (RWTH Aachen University)

Welcome and short introduction of SPP 2225

Session 1: How to study host cell exit? Examples of technical approaches

Chair: Mathias Hornef (University Hospital Aachen)

09:10 – 09:30 Julia Maria Post (University Medical Center Mainz – platform 1, Bindila lab)

Development of untargeted multiplex matrix assisted laser desorption ionization - ion mobility – high resolution mass spectrometry (MALDI-IM-HR-MS) - based assay for lipid biomarker discovery and quantitative lipidomics from biological samples.

09:30 – 09:50 Katherina Psathaki (Center of Cellular Nanoanalytics Osnabrück, - platform 2)

The iBiOs imaging platform for SPP 2225: Imaging modalities and recommended workflows

09:50 – 10:10 Freddy Frischknecht (University Hospital Heidelberg)

3D electron microscopy to understand parasite formation and egress

10:20 – 10:40 Özlem Günay-Esiyok (Humboldt University Berlin – Gupta lab)

Optogenetic induction of cyclic nucleotide signaling in Toxoplasma gondii

10:40 – 11:00 Elena Jimenez-Ruiz (Ludwig-Maximilians-University Munich – Meißner/Das lab)

A phenotypic screen using splitCas9 identifies essential genes required for actin regulation during host cell egress and invasion by Toxoplasma gondii

11:00 – 11:20 Michael Hensel University of Osnabrück)

Location, location, location: single cell approaches to investigate subcellular localization of intracellular pathogens

11:20 – 12:00 General discussion of session 1 topics

Session 2: The molecular machinery of host cell exit – part 1: the parasites

Chair: Freddy Frischknecht (University Hospital Heidelberg)

13:00 – 13:20 Katja Müller (Humboldt-University Berlin - Matuschewski lab)

Towards functional analysis of two essential Plasmodium berghei SERA proteases, SERA 3 and SERA 5, mediating host cell exit from erythrocytes and oocyst

13:20 – 13:40 Kai Matuschewski (Humboldt-University Berlin)

Evolutionary implications of synthetic viability of SERA1-3 triple knockout malarial parasites

13:40 – 14:00 Mattea Scheiner (Humboldt University Berlin - Ingmundson lab)

Plasmodium egress from the liver infection stage

14:10 – 14:30 Paul-Christian Burda (Centre for Structural Systems Biology Hamburg)

Identification and characterization of novel factors mediating Plasmodium falciparum egress from the red blood cell

14:30 – 14:50 Juliane Saßmannshausen (RWTH Aachen University – Pradel lab)

Unveiling the mechanisms of vesicle discharge during gametocyte egress of Plasmodium falciparum

14:50 – 15:10 Kim Chi Vo (Humboldt University Berlin – Gupta lab)

Phosphodiesterase-mediated counter-regulation of cyclic nucleotide signaling in Toxoplasma gondii

15:10 – 15:30 General discussion of session 2 topics

Friday, October 1, 2021:

Session 3: The molecular machinery of host cell exit – part 2: the bacteria

Chair: Sascha Brunke (Hans-Knöll-Institute Jena)

09:00 – 09:20 Monika Rangole (Research Center Borstel – Gutsmann lab)

Inside out - The role of mycobacterial ESX secretion systems in phagosome escape

09:20 – 09:40 Monica Hagedorn Jacobs University Bremen)

The ejectosome facilitates non-lytic egress of Mycobacterium marinum from the amoeba Dictyostelium discoideum

09:40 – 10:00 Sylvana Victoria Hüttel (University of Osnabrück - Barisch lab)

Role of mycobacterial (membrane) lipids on phagosomal escape of Mycobacterium marinum in Dictyostelium discoideum

10:10 – 10:30 Jana Scholz (Robert Koch Institute Berlin – Heuer lab Berlin)

Deciphering the role of sphingolipids in bacterial egress

10:30 – 10:50 Mathias Hornef (Institute of Medical Microbiology RWTH Aachen University Hospital )

SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella containing vacuoles in vivo

10:50 – 11:10 Xiao Liu (Eberhard Karls University Tübingen – Wolz/Weber lab)

LukAB is essential for Staphylococcus aureus exit from within macrophages

11:10 – 11:30 General discussion of session 3 topics

Session 4: The interplay between pathogens and host cells during exit

Chair: Christiane Wolz (Eberhard Karls University Tübingen)

12:30 – 12:50 Lydia Kasper & Theresa Rothe (Hans-Knöll-Institute Jena – Brunke/Hube lab)

Strategies of Candida albicans and Candida glabrata to exit from macrophages

12:50 – 13:10 Florens Lohrmann (University Medical Center Freiburg – Henneke lab)

Mycobacterial infection uncovers origin-dependent macrophage properties

13:10 – 13:30 Mohamed Balboul (University Hospital Erlangen – Lührmann lab)

Egress mechanisms of Coxiella burnetii

13:30 – 13:50 Jonathan Neuber (Robert Koch-Institute Wernigerode – Flieger lab)

Function of Legionella pneumophila phospholipases in vacuole and host egress

13:50 – 14:10 Iris Baars ( Otto-von-Guericke-University Magdeburg – Müller lab) & Moritz Jaedtka ( Paul-Ehrlich-Institute Langen – van Zandbergen lab)

Leishmania major dissemination among macrophage and monocytes populations in the skin

14:10 – 14:30 General discussion of session 4 topics & concluding remarks