Virtual project meeting Sep 30 - Oct 01, 2021
Our first content related virtual project meeting is now set for the 30th of September - 1st of October 2021. In the context of this meeting all project members will come together virtually to present their first results. Within the scope of this meeting, especially the young researchers (doctoral students, postdocs) shall be given the opportunity to present themselves and their projects to the community.
Programme:
Thursday, September 30, 2021:
09:00 – 09:10 Gabriele Pradel (RWTH Aachen University)
Welcome and short introduction of SPP 2225
Session 1: How to study host cell exit? Examples of technical approaches
Chair: Mathias Hornef (University Hospital Aachen)
09:10 – 09:30 Julia Maria Post (University Medical Center Mainz – platform 1, Bindila lab)
Development of untargeted multiplex matrix assisted laser desorption ionization - ion mobility – high resolution mass spectrometry (MALDI-IM-HR-MS) - based assay for lipid biomarker discovery and quantitative lipidomics from biological samples.
09:30 – 09:50 Katherina Psathaki (Center of Cellular Nanoanalytics Osnabrück, - platform 2)
The iBiOs imaging platform for SPP 2225: Imaging modalities and recommended workflows
09:50 – 10:10 Freddy Frischknecht (University Hospital Heidelberg)
3D electron microscopy to understand parasite formation and egress
10:20 – 10:40 Özlem Günay-Esiyok (Humboldt University Berlin – Gupta lab)
Optogenetic induction of cyclic nucleotide signaling in Toxoplasma gondii
10:40 – 11:00 Elena Jimenez-Ruiz (Ludwig-Maximilians-University Munich – Meißner/Das lab)
A phenotypic screen using splitCas9 identifies essential genes required for actin regulation during host cell egress and invasion by Toxoplasma gondii
11:00 – 11:20 Michael Hensel University of Osnabrück)
Location, location, location: single cell approaches to investigate subcellular localization of intracellular pathogens
11:20 – 12:00 General discussion of session 1 topics
Session 2: The molecular machinery of host cell exit – part 1: the parasites
Chair: Freddy Frischknecht (University Hospital Heidelberg)
13:00 – 13:20 Katja Müller (Humboldt-University Berlin - Matuschewski lab)
Towards functional analysis of two essential Plasmodium berghei SERA proteases, SERA 3 and SERA 5, mediating host cell exit from erythrocytes and oocyst
13:20 – 13:40 Kai Matuschewski (Humboldt-University Berlin)
Evolutionary implications of synthetic viability of SERA1-3 triple knockout malarial parasites
13:40 – 14:00 Mattea Scheiner (Humboldt University Berlin - Ingmundson lab)
Plasmodium egress from the liver infection stage
14:10 – 14:30 Paul-Christian Burda (Centre for Structural Systems Biology Hamburg)
Identification and characterization of novel factors mediating Plasmodium falciparum egress from the red blood cell
14:30 – 14:50 Juliane Saßmannshausen (RWTH Aachen University – Pradel lab)
Unveiling the mechanisms of vesicle discharge during gametocyte egress of Plasmodium falciparum
14:50 – 15:10 Kim Chi Vo (Humboldt University Berlin – Gupta lab)
Phosphodiesterase-mediated counter-regulation of cyclic nucleotide signaling in Toxoplasma gondii
15:10 – 15:30 General discussion of session 2 topics
Friday, October 1, 2021:
Session 3: The molecular machinery of host cell exit – part 2: the bacteria
Chair: Sascha Brunke (Hans-Knöll-Institute Jena)
09:00 – 09:20 Monika Rangole (Research Center Borstel – Gutsmann lab)
Inside out - The role of mycobacterial ESX secretion systems in phagosome escape
09:20 – 09:40 Monica Hagedorn Jacobs University Bremen)
The ejectosome facilitates non-lytic egress of Mycobacterium marinum from the amoeba Dictyostelium discoideum
09:40 – 10:00 Sylvana Victoria Hüttel (University of Osnabrück - Barisch lab)
Role of mycobacterial (membrane) lipids on phagosomal escape of Mycobacterium marinum in Dictyostelium discoideum
10:10 – 10:30 Jana Scholz (Robert Koch Institute Berlin – Heuer lab Berlin)
Deciphering the role of sphingolipids in bacterial egress
10:30 – 10:50 Mathias Hornef (Institute of Medical Microbiology RWTH Aachen University Hospital )
SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella containing vacuoles in vivo
10:50 – 11:10 Xiao Liu (Eberhard Karls University Tübingen – Wolz/Weber lab)
LukAB is essential for Staphylococcus aureus exit from within macrophages
11:10 – 11:30 General discussion of session 3 topics
Session 4: The interplay between pathogens and host cells during exit
Chair: Christiane Wolz (Eberhard Karls University Tübingen)
12:30 – 12:50 Lydia Kasper & Theresa Rothe (Hans-Knöll-Institute Jena – Brunke/Hube lab)
Strategies of Candida albicans and Candida glabrata to exit from macrophages
12:50 – 13:10 Florens Lohrmann (University Medical Center Freiburg – Henneke lab)
Mycobacterial infection uncovers origin-dependent macrophage properties
13:10 – 13:30 Mohamed Balboul (University Hospital Erlangen – Lührmann lab)
Egress mechanisms of Coxiella burnetii
13:30 – 13:50 Jonathan Neuber (Robert Koch-Institute Wernigerode – Flieger lab)
Function of Legionella pneumophila phospholipases in vacuole and host egress
13:50 – 14:10 Iris Baars ( Otto-von-Guericke-University Magdeburg – Müller lab) & Moritz Jaedtka ( Paul-Ehrlich-Institute Langen – van Zandbergen lab)
Leishmania major dissemination among macrophage and monocytes populations in the skin
14:10 – 14:30 General discussion of session 4 topics & concluding remarks