CV Caroline Barisch
Caroline Barisch studied biology at the University of Bayreuth and received her PhD from the University of Kassel in 2010. During her PhD in the lab of Prof. Markus Maniak she isolated lipid droplets from the model organism Dictyostelium discoideum and characterized the lipid droplet proteome.
In 2011, Caroline Barisch started her postdoc in the lab of Prof. Thierry Soldati at the University of Geneva, Switzerland. During the first years, she used the D. discoideum / M. marinum infection system to study lipid droplet dynamics during mycobacteria infection. 2016 she was promoted “maître assistante” and established several tools to monitor lipid flows from D. discoideum to intracellular mycobacteria. Besides lipid metabolism, Caroline Barisch also worked on zinc and ZnT transporters and their role in the bactericidal defense of D. discoideum and during mycobacteria infection.
In September 2019, Caroline Barisch started the junior research group Molecular Infection Biology at the University of Osnabrück, Germany. The main research interest of the group is to unravel the molecular mechanisms by which intracellular mycobacteria acquire host lipids. To this end, the lab is setting up metabolic tracing tools and mass spectrometry lipidomics. With the help of fluorescent and bifunctional lipid probes, her group will monitor the consequences of blocking specific lipid supply routes on various stages of the mycobacterial infection course.
- Niekamp K, Guzman G, Leier H, Rashidfarrokhi A, Richina V, Pott A, Barisch, C, Holthuis J, Tafesse F (2020) Sphingomyelin biosynthesis is essential for phagocytic signaling during Mycobacterium tuberculosis host cell entry. mBio 12:e03141-20 .
- Hanna N, Koliwer-Brandl H,Lefrançois LH, Kalinina V, Cardenal-Muñoz E, Appiah J, Leuba F, Hilbi H, *Soldati T, *Barisch C (2020) Zinc intoxication of Mycobacterium marinum during Dictyostelium discoideum infection is counteracted by induction of the pathogen Zn2+ exporter CtpC. mBio 12:e01313-20 .
- Knobloch P, Koliwer-Brandl H, Arnold FM, Hanna N, Gonda I, Adenau S, Personnic N, Barisch C, Seeger MA, Soldati T, Hilbi H (2020) Mycobacterium marinum produces distinct mycobactin and carboxymycobactin siderophores to promote growth in broth and phagocytes. Cell Microbiol 22:e13163.
- Luscher A, Fröhlich F, Barisch C, Littlewood C, Metcalfe J, Leuba F, Palma A, Pirruccello M, Cesareni G, Stagi M, Walther TC, Soldati T, De Camilli P, Swan LE (2019) Lowe syndrome-linked endocytic adaptors direct membrane cycling kinetics with OCRL in Dictyostelium discoideum . Mol Biol Cell 30:2268-2282.
- Koliwer-Brandl H, Knobloch P, Barisch C, Welin A, Hanna N, Soldati T, Hilbi H (2019) Distinct Mycobacterium marinum phosphatases determine pathogen vacuole phosphoinositide pattern, phagosome maturation, and escape to the cytosol. Cell Microbiol 21:e13008.
- López-Jiménez A T, Cardenal-Muñoz E, Leuba F, Gerstenmaier L, Barisch C, Hagedorn M., King JS, Soldati T (2018) The ESCRT and autophagy machineries cooperate to repair ESX-1-dependent damage at the Mycobacterium-containing vacuole but have opposite impact on containing the infection. PLoS Pathog 14:e1007501.
- B arisch, C, Kalinina V, Lefrançois LH, Appiah J, López-Jiménez AT, Soldati T (2018) Localization of all four ZnT zinc transporters in Dictyostelium and impact of ZntA and B knockout on bacteria killing. J Cell Sci 131:jcs222000.
- Sattler N, Bosmani C, Barisch C, Guého A, Gopaldass N, Dias M, Leuba F, Bruckert F, Cosson P, Soldati T (2018) Functions of the Dictyostelium LIMP-2 and CD36 homologues in bacteria uptake, phagolysosome biogenesis and host cell defence. J Cell Sci 131:jcs218040.
- Barisch C, Soldati T (2017) Breaking fat! How mycobacteria and other intracellular pathogens manipulate host lipid droplets. Rev Biochim 141:54-61.
- Barisch C, Soldati T (2017) Mycobacterium marinum degrades both triacylglycerols and phospholipids from its Dictyostelium host to synthesise its own triacylglycerols and generate lipid inclusions. PLoS Pathog 13:e1006095.