Publications

 

The following publications have hitherto been published within the SPP 2225 (names of PIs and SPP 2225-funded scientists in bold; publications directly funded by the SPP 2225 are marked with an asterisk; publications as part of the Molecular Microbiology Special Issue: Mechanisms of host cell exit by intracellular pathogens are marked with a hashtag):

2023

Scheiner M, Burda PC, Ingmundson A (2023)*# Moving on: How malaria parasites exit the liver. Mol Microbiol, in press. doi: 10.1111/mmi.15141

Volkmar K*, Jaedtka M*, Baars I, Walber B, Philipp S, Bagola K, Müller AJ, Heine H, van Zandbergen G (2023)#* Investigating pyroptosis as a mechanism of L. major cell-to-cell spread in the human BLaER1 infection model. Mol Microbiol, in press.

Hiller M, Diwo M, Wamp S, Gutsmann T, Lang C, Blankenfeldt W, Flieger A (2023)* Structure-function relationships underpin disulfide loop cleavage-dependent activation of Legionella pneumophila lysophosholipase A PlaA. bioRxiv doi: https://doi.org/10.1101/2023.03.24.534060

Farrukh A, Musabyimana JP, Distler U, Mahlich VJ, Mueller J, Bick F, Tenzer S, Pradel G, Ngwa CJ (2023)*# The Plasmodium falciparum CCCH zinc finger protein MD3 regulates male gametocytogenesis through its interaction with RNA-binding proteins. bioRxiv doi: https://doi.org/10.1101/2023.07.19.549485

Distler U, Łącki MK, Startek MP, Teschner D, Sven Brehmer, Decker J, Schild T, Krieger J, Krohs F, Raether O, Hildebrandt A, Tenzer S (2023) midiaPASEF maximizes information content in data-independent acquisition proteomics. bioRxiv doi: doi: https://doi.org/10.1101/2023.01.30.526204

Lange T, Kasper L, Gresnigt MS, Brunke S, Hube B (2023)* “Under Pressure" - How fungi evade, exploit, and modulate cells of the innate immune system. Semin Immunol 66:101738. doi: 10.1016/j.smim.2023.101738

Listian SA, Kol M, Ufelmann E, Eising S, Froehlich F, Walter S, Holthuis JCM, Barisch C (2023)* Complex sphingolipid profiling and identification of an inositol phosphorylceramide synthase in Dictyostelium discoideum. bioRxiv 2023.07.07.548115; doi: https://doi.org/10.1101/2023.07.07.548115

Baars I, Jaedtka M, Dewitz LA, Fu Y, Franz T, Mohr J, Gintschel P, Berlin H, Degen A, Freier S, Rygol SM, Schraven B, Kahlfuss S, van Zandbergen G, Müller AJ (2023)* Leishmania major drives host phagocyte death and cell-to-cell transfer depending on intracellular pathogen proliferation rate. JCI Insight 13:e169020. doi: 10.1172/jci.insight.169020

Gerstenmaier L, Colasanti O, Behrens H, Kolonko M, Hammann C, Hagedorn M (2023)*# Recruitment of both the ESCRT and autophagic machineries to ejecting Mycobacterium marinum. Mol Microbiol, in press. doi: 10.1111/mmi.15075

Scharte F, Franzkoch R, Hensel M (2023)*# Flagella-mediated cytosolic motility of Salmonella enterica Paratyphi A aids in evasion of xenophagy but does not impact egress from host cells. Mol Microbiol, in press. doi: 10.1111/mmi.15104

Franzkoch R, Anand A, Breitsprecher L, Psathaki OE, Barisch C (2023)* Resolving exit strategies of mycobacteria by combining high-pressure freezing with 3D-correlative light and electron microscopy bioRxiv preprint; https://doi.org/10.1101/2023.04.24.538041.

Barisch C, Holthuis JCM, Cosentino C (2023)* Membrane damage and repair: a thin line between life and death. Biol Chem; https://doi.org/10.1515/hsz-2022-0321.

Anand A, Mazur AC, Rosell-Arevalo P, Franzkoch R, Breitsprecher L, Listian SA, Hüttel SV, Müller D, Schäfer DG, Vormittag S, Hilbi H, Maniak M, Gutierrez MG, Barisch C (2023)* ER-dependent membrane repair of mycobacteria-induced vacuole damage. mBio, in press.

Scharte F, Franzkoch R, Hensel M (2023)* From vacuole to cytosol – Disruptive invasion triggers cytosolic release of Salmonella Paratyphi A and subsequent cytosolic motility favors evasion of xenophagy. bioRxiv preprint; doi: https://doi.org/10.1101/2022.07.24.501230.

Hiller M, Diwo M, Wamp S, Gutsmann T, Lang C, Blankenfeldt W, Flieger A (2023)* Structure-function relationships underpin disulfide loop cleavage-dependent activation of Legionella pneumophila lysophosholipase A PlaA. bioRxiv preprint; https://doi.org/10.1101/2023.03.24.534060.

Chen K, Huang X, Distler U, Tenzer S, Günay-Esiyok Ö, Gupta N (2023)* Apically-located P4-ATPase1-Lem1 complex internalizes phosphatidylserine and regulates motility-dependent invasion and egress in Toxoplasma gondii. Comput Struct Biotechnol J 21:1893-1906.

Lerner, R., Baker, D., Schwitter, C. Neuhaus S, Hauptman T, Post JM, Kramer S, Bindila L (2023)* Four-dimensional trapped ion mobility spectrometry lipidomics for high throughput clinical profiling of human blood samples. Nat Commun 14:937.

Frischknecht F, Enninga J (2023)* Actin subversion for productive Plasmodium hepatocyte invasion. Trends Parasitol; https://doi.org/10.1016/j.pt.2023.03.003.

Ferreira JL, Pražák V, Vasishtan D, Siggel M, Hentzschel F, Binder AM, Pietsch E, Kosinski J, Frischknecht F, Gilberger TW, Grünewald K (2023)* Variable microtubule architecture in the malaria parasite. Nat Comm; https://doi.org/10.1038/s41467-023-36627-5.

Farrukh A, Musabyimana JP, Distler U, Tenzer S, Pradel G, Ngwa CJ (2023)* Proximity interaction analysis of the Plasmodium falciparum 1 putative ubiquitin ligase PfRNF1 reveals a role in RNA regulation. bioRxiv preprint; https://doi.org/10.1101/2023.03.16.533063.

Sassmannshausen J, Bennink S, Distler U, Küchenhoff J, Minns AM, Lindner SE, Burda PC, Tenzer S, Gilberger TW, Pradel G (2023)*# Comparative proteomics of vesicles essential for the egress of Plasmodium falciparum gametocytes from red blood cells. Mol Microbiol, in press. doi: 10.1111/mmi.15125

Singer M, Simon K, Forné I, Meissner M (2023)* A central CRMP complex essential for invasion in Toxoplasma gondii. PLoS Biol 21:e3001937.

Wichers-Misterek JS, Binder AM, Mesén-Ramírez P, Dorner LP, Safavi S, Fuchs G, Lenz TL, Bachmann A, Wilson D, Frischknecht F, Gilberger TW (2023)* A Microtubule- associated protein is essential for malaria parasite transmission. mBio 14:e0331822.

Mauermeir M, Ölke M, Hayek I, Schulze-Luehrmann J, Dettmer K, Oefner PJ, Berens C, Menge C, Lührmann A (2023) Bovine blood derived macrophages are unable to control Coxiella burnetii replication under hypoxic conditions. Front Immunol 14:960927.

Vormittag S, Hüsler D, Haneburger I, Kroniger T, Anand A, Prantl M, Barisch C, Maaß S, Becher D, Letourneur F, Hilbi H (2023) Legionella- and host-driven lipid flux at LCV-ER membrane contact sites promotes vacuole remodeling. EMBO Rep 24:e56007.

2022

Niekamp P, Scharte F, Sokoya T, Vittadello L, Kim Y, Deng Y, Südhoff E, Hilderink A, Imlau M, Clarke CJ, Hensel M, Burd CG, Holthuis JCM (2022)* Ca2+-activated sphingomyelin scrambling and turnover mediate ESCRT-independent lysosomal repair. Nat Commun 13:1875.

Post JM, Lerner R, Schwitter C, Lutz B, Lomazzo E, Bindila L (2022)* Lipidomics and Transcriptomics in Neurological Diseases. J Vis Exp 181:e59423.

Alder A, Wilcke L, Pietsch E, von Thien H, Pazicky S, Löw C, Mesen-Ramirez P, Bachmann A, Burda PC, Kunick C, Sondermann H, Wilson D, Gilberger TW (2022)* Functional inactivation of Plasmodium falciparum glycogen synthase kinase GSK3 modulates erythrocyte invasion and blocks gametocyte maturation. J Biol Chem 298:102360.

Burian M, Wolz C, Yazdi AS (2022)* Transcriptional adaptation of staphylococci during colonization of the authentic human environment: An overview of transcriptomic changes and their relationship to physiological conditions. Front Cell Infect Microbiol 12:1062329.

Mogavero S, Höfs S, Lauer AN, Müller R, Brunke S, Allert S, Gerwien F, Groth S, Dolk E, Wilson D, Gutsmann T, Hube B (2022)* Candidalysin Is the Hemolytic Factor of Candida albicans. Toxins (Basel) 14:874.

Lagune M, Le Moigne V, Johansen MD, Vásquez Sotomayor F, Daher W, Petit C, Cosentino G, Paulowski L, Gutsmann T , Wilmanns M, Maurer FP, Herrmann JL, Girard-Misguich F, Kremer L. (2022)* The ESX-4 substrates, EsxU and EsxT, modulate Mycobacterium abscessus fitness. PLoS Pathog 18:e1010771.

Gabelich JA, Grützke J, Kirscht F, Popp O, Matz JM, Dittmar G, Rug M, Ingmundson A (2022) A member of the tryptophan-rich protein family is required for efficient sequestration of Plasmodium berghei schizonts. PLoS Pathog 18:e1010846.

Vo KC, Ruga L, Psathaki OE, Franzkoch R, Distler U, Tenzer S, Hensel M, Hegemann P, Gupta N (2022)* Plasticity and therapeutic potential of cAMP and cGMP-specific phosphodiesterases in Toxoplasma gondii. Comput Struct Biotechnol J 20:5775-5789.

Sprague, JL, Kasper L, Hube B (2022)* From intestinal colonization to systemic infections: Candida albicans translocation and dissemination. Gut Microbes, 14:2154548.

Alder A, Wilcke L, Pietsch E, von Thien H, Pazicky S, Löw C, Mesen-Ramirez P, Bachmann A, Burda PC, Kunick C, Sondermann H, Wilson D, Gilberger TW (2022)* Functional inactivation of Plasmodium falciparum glycogen synthase kinase GSK3 modulates erythrocyte invasion and blocks gametocyte maturation. J Biol Chem 298:102360.

Musabyimana JP, Distler U, Sassmannshausen J, Berks C, Manti J, Bennink S, Blaschke L, Burda PC, Flammersfeld A, Tenzer S, Ngwa CJ, Pradel G (2022)* Plasmodium falciparum S-Adenosylmethionine Synthetase Is Essential for Parasite Survival through a Complex Interaction Network with Cytoplasmic and Nuclear Proteins. Microorganisms 10:1419.

Munson GP, Gilbert RJC, Pradel G (2022)* Editorial: Perforins and Cholesterol-Dependent Cytolysins in Immunity and Pathogenesis. Front Immunol 13:932502.

Roling L, Flammersfeld A, Pradel G, Bennink S (2022) The WD40-Protein PfWLP1 Ensures Stability of the PfCCp-Based Adhesion Protein Complex in Plasmodium falciparum Gametocytes. Front Cell Infect Microbiol 12:942364.

Kimmel J, Kehrer J, Frischknecht F, Spielmann T (2022)* Proximity-dependent biotinylation approaches to study apicomplexan biology. Mol Microbiol117 :553-568.

Ripp J, Smyrnakou X, Neuhoff MT, Hentzschel F, Frischknecht F (2022)* Phosphorylation of myosin A regulates gliding motility and is essential for Plasmodium transmission. EMBO Rep 23:e54857.

Hentzschel F, Frischknecht F (2022)* Still enigmatic: Plasmodium oocysts 125 years after their discovery. Trends Parasitol 38:610-613.

Li W, Grech J, Stortz JF, Gow M, Periz J, Meissner M, Jimenez-Ruiz E (2022)* A splitCas9 phenotypic screen in Toxoplasma gondii identifies proteins involved in host cell egress and invasion. Nat Microbiol 7:882-895.

Siscar-Lewin S, Hube B, Brunke S (2022)* Emergence and evolution of virulence in human pathogenic fungi. Trends Microbiol 30:693-704.

Westman J, Plumb J, Licht A, Yang M, Allert S, Naglik JR, Hube B, Grinstein S, Maxson ME (2022)* Calcium-dependent ESCRT recruitment and lysosome exocytosis maintain epithelial integrity during Candida albicans invasion.Cell Rep 38:110187.

Cordsmeier A, Rinkel S, Jeninga M, Schulze-Luehrmann J, Ölke M, Schmid B, Hasler D, Meister G, Häcker G, Petter M, Beare PA, Lührmann A (2022) The Coxiella burnetii T4SS effector protein AnkG hijacks the 7SK small nuclear ribonucleoprotein complex for reprogramming host cell transcription. PLoS Pathog 18:e1010266.

Prodjinotho UF, Gres V, Henkel F, Lacorcia M , Dandl R, Haslbeck M , Schmidt V , Winkler AS, Sikasunge C, Jakobsson PJ, Henneke P, Esservon Bieren J, Prazeres da Costa C (2022)* Helminthic dehydrogenase drives PGE2 and IL-10 production in monocytes to potentiate Treg induction. EMBO Rep 23:e54096.

2021

Matsumoto M, Nakagawa S, Zhang L, Nakamura Y, Villaruz AE, Otto M, Wolz C, Inohara N, Nunez G (2021) Interaction between Staphylococcus Agr virulence and neutrophils regulates pathogen expansion in the skin. Cell Host Microbe 29:930-940.

Striednig B, Lanner U, Niggli S, Katic A, Vormittag S, Brülisauer S, Hochstrasser R, Kaech A, Welin A, Flieger A, Ziegler U, Schmidt A, Hilbi H, Personnic N (2021) Quorum sensing governs a transmissive Legionella subpopulation at the pathogen vacuole periphery EMBO Reports 22:e52972.

Formaglio P, Alabdullah M, Siokis A, Handschuh J, Sauerland I, Fu Y, Krone A, Gintschel P, Stettin J, Heyde S, Mohr J, Philipsen L, Schröder A, Robert PA, Zhao G, Khailaie S, Dudeck A, Bertrand J, Späth GF, Kahlfuß S, Bousso P, Schraven B, Huehn J, Binder S, Meyer-Hermann M, Müller AJ (2021) Nitric oxide controls proliferation of Leishmania major by inhibiting the recruitment of permissive host cells. Immunity 54:2724-2739.

Reuter T, Scharte F, Franzkoch R, Liss V, Hensel M (2021) Single cell analyses reveal distinct adaptation of typhoidal and non-typhoidal Salmonella enterica serovars to intracellular lifestyle. PLoS Pathog 17:e1009319.

Röder J, Felgner P, Hensel M (2021) Single-cell analyses reveal phosphate availability as critical factor for nutrition of Salmonella enterica within mammalian host cells. Cell Microbiol 23:e13374.

Röder J, Felgner P, Hensel M (2021) Comprehensive single-cell analyses of the nutritional environment of intracellular Salmonella enterica. Front Cell Infect Microbiol 11:624650.

Schulte M, Olschewski K, Hensel M (2021) Fluorescent protein-based reporters reveal stress response of intracellular Salmonella enterica at level of single bacterial cells. Cell Microbiol 23:e13293.

Schulte M, Olschewski K, Hensel M (2021) The protected physiological state of intracellular Salmonella enterica persisters reduces host cell-imposed stress. Commun Biol 4:520.

Wagner C, Torow N, Hornef MW, Lelouard H (2021)* Spatial and temporal key steps in early-life intestinal immune system development and education. FEBS J 289:4731-4757.

Henneke P, Kierdorf K, Hall LJ, Sperandio M, Hornef M (2021)* Perinatal development of innate immune topology. Elife 10:e67793.

Diwo M, Michel W, Aurass P, Kuhle-Keindorf K, Pippel J, Krausze J, Wamp S, Lang C, Blankenfeldt W, Flieger A (2021) NAD(H)-mediated tetramerization controls the activity of Legionella pneumophila phospholipase PlaB. Proc Natl Acad Sci U S A 118:e2017046118.

Moghadam ZM, Henneke P, Kolter J (2021) From Flies to Men: ROS and the NADPH Oxidase in Phagocytes. Front Cell Dev Biol 9:628991.

Lösslein AK, Henneke P (2021) Mycobacterial immunevasion-Spotlight on the enemy within. J Leukoc Biol 109:9-11.

Lösslein AK, Lohrmann F, Scheuermann L, Gharun K, Neuber J, Kolter J, Forde AJ, Kleimeyer C, Poh YY, Mack M, Triantafyllopoulou A, Dunlap MD, Khader SA, Seidl M, Hölscher A, Hölscher C, Guan XL, Dorhoi A, Henneke P (2021)* Monocyte progenitors give rise to multinucleated giant cells. Nat Commun 12:2027.

Fulde M, van Vorst K, Zhang K, Westermann AJ, Busche T, Huei YC, Welitschanski K, Froh I, Pägelow D, Plendl J, Pfarrer C, Kalinowski J, Vogel J, Valentin-Weigand P, Hensel M, Tedin K, Repnik U, Hornef MW (2021) SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella-containing vacuoles in vivo . Gut Microbes 13:1973836.

Singer M, Frischknecht F (2021) Fluorescent tagging of Plasmodium circumsporozoite protein allows imaging of sporozoite formation but blocks egress from oocysts. Cell Microbiol 23:e13321.

Radosa S, Sprague JL, Lau SH, Tóth R, Linde J, Krüger T, Sprenger M, Kasper L, Westermann M, Kniemeyer O, Hube B, Brakhage AA, Gácser A, Hillmann F (2021) The fungivorous amoeba Protostelium aurantium targets redox homeostasis and cell wall integrity during intracellular killing of Candida parapsilosis .Cell Microbiol 23:e13389.

Fulde M, van Vorst K, Zhang K, Westermann A J, Busche T, Huei Y C, Welitschanski K, Froh I, Pägelow D, Plendl J, Pfarrer C, Kalinowski J, Vogel J, Valentin-Weigand P, Hensel M, Tedin K, Repnik U, Hornef MW (2021) SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella-containing vacuoles in vivo. Gut Microbes 13:1973836.

Bennink S, Pradel G (2021)* Vesicle dynamics during the egress of malaria gametocytes from the red blood cell. Mol Biochem Parasitol 243:111372.

Friedrich A, Beare PA, Schulze-Luehrmann J, Cordsmeier A, Pazen T, Sonnewald S, Lührmann A (2021) The Coxiella burnetii effector protein CaeB modulates endoplasmatic reticulum (ER) stress signalling and is required for efficient replication in Galleria mellonella. Cell Microbiol 23:e13305.

Striednig B, Lanner U, Niggli S, Katic A, Vormittag S, Brülisauer S, Hochstrasser R, Kaech A, Welin A, Flieger A, Ziegler U, Schmidt A, Hilbi H, Personnic N (2021) Quorum sensing governs a transmissive Legionella subpopulation at the pathogen vacuole periphery. EMBO Rep 27:e52972.

Kreutzfeld O, Grützke J, Ingmundson A, Müller K, Matuschewski K (2021) Absence of PEXEL-Dependent Protein Export in Plasmodium Liver Stages Cannot Be Restored by Gain of the HSP101 Protein Translocon ATPase. Front Genet 12:742153.

Hanna N, Koliwer-Brandl H, Lefrançois LH, Kalinina V, Cardenal-Muñoz E, Appiah J, Leuba F, Gueho A, Hilbi H, Soldati T, Barisch C (2021) Zn²⁺ Intoxication of Mycobacterium marinum during Dictyostelium discoideum Infection Is Counteracted by Induction of the Pathogen Zn²⁺ Exporter CtpC. mBio 12:e01313-20.

Baasch S , Giansanti P , Kolter J , Riedl A , Forde AJ , Runge S, Zenke S , Elling R , Halenius A , Brabletz S , Hengel H , Kuster B, Brabletz T , Cicin-Sain L , Arens R , Vlachos A , Rohr JC , Stemmler MP , Kopf M , Ruzsics Z, Henneke P (2021)* Cytomegalovirus subverts macrophage identity Cell 184:3774-3793.

2020

Vo KC, Günay-Esiyok Ö, Liem N, Gupta N (2020) The protozoan parasite Toxoplasma gondii encodes a gamut of phosphodiesterases during its lytic cycle in human cells. Comput Struct Biotechnol J 18:3861-3876.

Putrianti ED, Schmidt-Christensen A, Heussler V, Matuschewski K, Ingmundson A (2020) A Plasmodium cysteine protease required for efficient transition from the liver infection stage. PLoS Pathog 16:e1008891.

Röder J, Hensel M (2020) Presence of SopE and mode of infection result in increased Salmonella-containing vacuole damage and cytosolic release during host cell infection by Salmonella enterica. Cell Microbiol 22:e13155.

Bennink S, Pradel G (2020) Exit - Wirtszellaustritt intazellulärer Pathogene. Biospektrum 26:478-481.

Westman J, Walpole GWF, Kasper L, Xue BY, Elshafee O, Hube B, Grinstein S. (2020) Lysosome fusion maintains phagosome integrity during fungal infection . Cell Host Microbe 28:798-812.

Liffner B., Frölich S., Heinemann GK., Liu B., Dixon MWA., Gilberger TW, Wilson DW (2020) PfCERLI1 is a covered rhoptry associated protein essential for Plasmodium falciparum merozoite invasion of erythrocytes. Nat Commun 11:1411.

Bitschar K, Staudenmaier L, Klink L, Focken J, Sauer B, Fehrenbacher B, Herster F, Bittner Z, Bleul L, Schaller M, Wolz C, Weber ANR, Peschel A, Schittek B (2020) Staphylococcus aureus Skin Colonization Is Enhanced by the Interaction of Neutrophil Extracellular Traps with Keratinocytes. J Invest Dermatol 140:1054-1065.

Burda PC, Crosskey T, Lauk K, Zurborg A, Söhnchen C, Liffner B, Wilcke L, Pietsch E, Strauss J, Jeffries CM, Svergun DI, Wilson DW, Wilmanns M, Gilberger TW (2020) Structure- Based Identification and Functional Characterization of a Lipocalin in the Malaria Parasite Plasmodium falciparum . Cell Rep 31:107817.

Sassmannshausen J, Pradel G, Bennink S (2020) Perforin-Like Proteins of Apicomplexan Parasites. Front Cell Infect Microbiol 10:578883.

Sprenger M, Hartung TS, Allert S, Wisgott S, Niemiec MJ, Graf K, Jacobsen ID, Kasper L, Hube B (2020). Fungal biotin homeostasis is essential for immune evasion after macrophage phagocytosis and virulence. Cell Microbiol 22:e13197.

Pechstein J, Schulze-Luehrmann J, Bisle S, Cantet F, Beare PA, Ölke M, Bonazzi M, Berens C, Lührmann A (2020) The Coxiella burnetii T4SS Effector AnkF Is Important for Intracellular Replication. Front Cell Infect Microbiol 10:559915.

2018

Flieger A, Frischknecht F, Häcker G, Hornef MW, Pradel G (2018) Pathways of host cell exit by intracellular pathogens. Microb Cell 5:525-544.

Haas A, Hensel M, Lührmann A, Rudel T, Saftig P, Schaible UE (2018) Intracellular compartments of pathogens: Highways to hell or stairways to heaven? Int J Med Microbiol 308:1-2.

Westman J, Moran G, Mogavero S, Hube B, Grinstein S (2018) Candida albicans Hyphal Expansion Causes Phagosomal Membrane Damage and Luminal Alkalinization. mBio 9:e01226-18.